This does not alter our adherence to PLOS ONE policies on sharing data and materials.Īlthough most preclinical studies show promising results for stem cell therapy in various small animal models of stroke, randomized trials of stem cell therapy in stroke patients show negative or mixed results. There are no patents, products in development or marketing products to declare. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have read the journal’s policy and the authors of this manuscript have the following competing interests: JHS, EHK, and OYB are paid employees of S&E bio, Inc. The specific roles of these authors are articulated in the ‘author contributions’ section. provided support for this study in the form of salaries for JHS, EHK, and OYB. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript and its Supporting information files.įunding: This study was supported by a grant from the Korea Health Technology R&D Project, the Ministry of Health & Welfare (HI14C16240000 and HI14C3484). Received: OctoAccepted: JanuPublished: February 16, 2021Ĭopyright: © 2021 Son et al. PLoS ONE 16(2):Įditor: Quan Jiang, Henry Ford Health System, UNITED STATES (2021) Brain morphological and connectivity changes on MRI after stem cell therapy in a rat stroke model. These quantitative MRI measurements could be potential outcome predictors of functional recovery after treatment with stem cells for stroke.Ĭitation: Son JP, Sung JH, Kim DH, Cho YH, Kim SJ, Chung J-W, et al. T 2-weighted volume alterations (ischemic lesion and brain atrophy), and DTI microstructural indices and rFD changes, were well matched with the results of behavioral assessment. In DTI, rFA and rFD values were significantly higher and rRD value was significant lower in the SS-hMSCs group and these microstructure/connectivity changes were correlated with T 2-weighted morphological changes. Brain atrophy was significantly decreased in the SS-hMSCs group compared to the other groups. Infarct lesion volume of the SS-hMSCs group was significantly decreased at 2 weeks when compared to the PBS-only groups, but there were no differences between the FBS-hMSCs and SS-hMSCs groups. According to mNSS results, the SS-hMSCs group showed the most prominent functional improvement. Brain microstructure/connectivity changes were evaluated in the ischemic recovery area by DTI-derived microstructural indices such as relative fractional anisotropy (rFA), relative axial diffusivity (rAD), and relative radial diffusivity (rRD), and relative fiber density (rFD) analyses. Quantitative analyses of T 2-weighted ischemic lesion and ventricular volume changes were performed. Functional improvement was assessed using a modified neurological severity score (mNSS). Transient middle cerebral artery occlusion rats randomly received PBS (PBS-only), FBS cultured hMSCs (FBS-hMSCs), or stroke patients’ serum cultured hMSCs (SS-hMSCs). We evaluated morphological and connectivity changes after treatment with human mesenchymal stem cells (hMSCs) in a rat stroke model, through quantitative measurement of T 2-weighted images and diffusion tensor imaging (DTI). In animal models of stroke, behavioral assessments could be complemented by a variety of neuroimaging studies to correlate them with recovery and better understand mechanisms of improvement after stem cell therapy.
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